Matthias Ugele
Siemens Healthcare, Germany
Title: Label-free leukemia differentiation with high-throughput digital holographic microscopy
Biography:
Abstract:
Blood smear analysis is the gold standard for routine diagnosis of hematological disorders and serves as recommendation for
further differential diagnosis such as flow cytometric immunophenotyping. Analysis and interpretation of peripheral blood
smears is time consuming and depends on interobserver variation. Most recently, label-free imaging based on Digital Holographic
Microscopy (DHM) emerged with the potential to perform label-free hematology analysis. Here, we present label-free leukemia
differentiation of clinical samples using a differential DHM in combination with three-dimensional hydrodynamic focusing of blood
cells. Based on principal component analysis and physical parameters we developed a gating strategy for the differentiation of 10
leukocyte subtypes which enabled the differentiation of Acute Myeloid Leukemia (AML), Acute Lymphocytic Leukemia (ALL),
Chronic Lymphocytic Leukemia (CLL) and Myelo Proliferative Neoplasms (MPN). Furthermore, we observed the possibility of MPN
subtyping and demonstrated in a one case study the progression of AML from first diagnosis to remission.